Journal: Cells

Article Title: Sclerostin Alters Tumor Cell Characteristics of Oral Squamous Cell Carcinoma and May Be a Key Player in Local Bone Invasion

doi: 10.3390/cells13020137

Figure Lengend Snippet: Immunohistochemical staining protocol.

Article Snippet: Sclerostin , Mouse, monoclonal, clone AbD09097_h/mIgG 2a, 1:1200 , HIER (pH 9) , Dako EnVision FLEX , BioRad, Hercules, CA, USA (HCA230Z).

Techniques: Immunohistochemical staining, Staining

Journal: International Journal of Molecular Medicine

Article Title: Icaritin promotes the osteogenesis of bone marrow mesenchymal stem cells via the regulation of sclerostin expression

doi: 10.3892/ijmm.2020.4470

Figure Lengend Snippet: Icaritin (1 µ M) increases mRNA levels of osteogenic marker genes. (A) SOST , (B) OCN , (C) Runx2 , (D) Alp and (E) β-catenin at the different time points of osteogenesis of human bone marrow-derived mesenchymal stem cells. DMSO was used as the control group. Data are presented as the mean ± standard deviation (n=3). * P<0.05 and ** P<0.01 vs. control group at the same stage. SOST, sclerostin; OCN, osteocalcin; Runx2, RUNX family transcription factor 2; Alp, alkaline phosphatase.

Article Snippet: The membrane was incubated with antibodies targeting β-actin (1:1,000; cat. no. 4970S; Cell Signaling Technology, Inc.), osteocalcin (OCN; 1:1,000; cat. no. MAB1419; R&D Systems, Inc.), RUNX family transcription factor 2 (Runx2; 1:1,000; cat. no. 12556; Cell Signaling Technology, Inc.), ALP (1:1,000; cat. no. AF2910; R&D Systems, Inc.), β-catenin (1:1,000; cat. no. 8480S; Cell Signaling Technology, Inc.) and SOST (1:1,000; cat. no. MAB1406; R&D Systems, Inc.) overnight at 4°C.

Techniques: Marker, Derivative Assay, Control, Standard Deviation

Journal: International Journal of Molecular Medicine

Article Title: Icaritin promotes the osteogenesis of bone marrow mesenchymal stem cells via the regulation of sclerostin expression

doi: 10.3892/ijmm.2020.4470

Figure Lengend Snippet: SOST overexpression reverses icaritin-induced osteogenesis of hBMSCs. (A) Western blot analysis for the protein level of SOST. (B) The mRNA expression of SOST in BMSCs, BMSCs-vector, and BMSCs-SOST. (C) Mineralization in cultured hBMSCs in BMSCs-vector and BMSCs-SOST groups with or without icaritin were detected at day 14. Magnification, ×10. (D) The mRNA levels of OCN, Runx2, Alp and β-actin were determined by reverse transcription quantitative polymerase chain reaction. Data are presented as mean ± standard deviation (n=3). ** P<0.01 vs. BMSCs group; # P<0.05 and ## P<0.01 vs. BMSCs-vector group. SOST, sclerostin; BMSCs, bone marrow-derived mesenchymal stem cells; OCN, osteocalcin; Runx2, RUNX family transcription factor 2; Alp, alkaline phosphatase.

Article Snippet: The membrane was incubated with antibodies targeting β-actin (1:1,000; cat. no. 4970S; Cell Signaling Technology, Inc.), osteocalcin (OCN; 1:1,000; cat. no. MAB1419; R&D Systems, Inc.), RUNX family transcription factor 2 (Runx2; 1:1,000; cat. no. 12556; Cell Signaling Technology, Inc.), ALP (1:1,000; cat. no. AF2910; R&D Systems, Inc.), β-catenin (1:1,000; cat. no. 8480S; Cell Signaling Technology, Inc.) and SOST (1:1,000; cat. no. MAB1406; R&D Systems, Inc.) overnight at 4°C.

Techniques: Over Expression, Western Blot, Expressing, Plasmid Preparation, Cell Culture, Reverse Transcription, Real-time Polymerase Chain Reaction, Standard Deviation, Derivative Assay

Journal: Clinical Journal of the American Society of Nephrology

Article Title: Sclerostin and Dickkopf-1 in Renal Osteodystrophy

doi: 10.2215/cjn.06550810

Figure Lengend Snippet: Figure 1. | (A and B) Scatter plot of serum concentrations of sclerostin (pg/ml) at different levels of Ac.f. (A) and different levels of BFR/BS (B).

Article Snippet: Fifty microliters of serum was loaded per well, incubated overnight at 4°C, washed, and incubated for 1 hour at 37°C, followed by incubation at 4°C for 1 hour with a biotinylated polyclonal anti-sclerostin antibody (BAF 1406; R&D Systems) diluted to a concentration of 0.5 g/ml in dilution buffer.

Techniques: